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KMID : 0350519940470041869
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1869 ~ p.1879
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Interphase Cytogenetics of Non-Hodgkin's Lymphoma Using Non-Fluorescent in Situ Hybridization in Paraffin embedded Tissue
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Abstract
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Non-Hodgkin's lymphoma (NHL) consists of a heterogeneous group of tumors and this can be accounted from various histology, immunology and clinical findings of this tumor. By the development of molecular biology, especially analysis of DNA content
and
ploidy, it is generally accepted that the aneuploidy is closely related with malignancy. The conventional cytogenetic investigations have demonstrated that the majority of NHL have various clonal karyotypic abnormalities. But these techniques
require
metaphase spread by in vitro culture of tumor cells and have limitations in determining the major clone of tumor cells. Recently, in situ hybridization (ISH) of interphase cells has used for the cytogenetic study in paraffin-embedded solid
tumors.
We performed interphase cytogenetic study of 30 cases of NHL and 10 of reactive hyperplasia by ISH using non-fluorescent enzymatic method. The results of cytogenetic study was analyzed and compared with the histopathologic and immunologic
subtypes
of
NHL.
1. In reactive hyperplasia. All were diploid for all four chromosomes, X, 7, 12 and 18.
2. Aberrant chromosomes were observed in 27 (90%) out of 30 NHL cases. The changes of chromosome number were most frequently found in chromosome 7 (13 cases) followed by chromosome X and 18 (12 cases, respectively. The extracopy of X chromosome
was the
most commonly observed aberration (14 cases). The translocation of chromosome 18 was observed in 5 cases (16.7%).
3. Some characteristic chromosomal aberrations were found in each subtypes and grades of NHI. In low grade NHL (9 cases), the extracopy of chromosome 12 was found in 3 cases (33.3%), but extracopy of chromosome 7 or 18 was not observed. Disomy X
was
frequently found in 3 out of 4 cases of small lymphocytic lymphoma (75%). In intermediate grade (16 cases), tetraploidy was characteristically found in 4 cases (25%), and extracopy of chromosome 18 was frequently observed in diffuse large cell
lymphoma.
Among total 5 cases of translocation involving chromosome 18, four were detected in intermediate grade NHL and 3 cases of them were diffuse, large noncleaved cell lymphoma. In high grade (5 cases), there were no more specific chromosomal
aberrations
than other grades.
4. Immunophenotypical difference of cytogenetic aberrations was not characteristic, but polysomy X and trisomy 18 were detected only in B cell NHL, while aberration of chromosome 7 was not found in T cell NHL.
In conclusion, we demonstrated the numerical and structural chromosomal aberrations in NHL using non-fluorescent in situ hybridization and oberved the possible role of chromosomal aberrations in the pathogenesis of NHL. And the correlation of
some
specific chromosomal aberrations with histopathologic subtypes and grades suggest that the cytogenetic analysis by ISH could provide us more valuable for pathologic diagnosis and clinical outcome of NHL.
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KEYWORD
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